PDBsum entry 9hgo

Cytosolic protein

PDB id: 9hgo

Contents

Protein chains

298 a.a.

Ligands

EDO ×8

Metals

_CL ×2

Waters ×238

PDB id:

9hgo

Name:

Cytosolic protein

Title:

Crystal structure of the coxiella burnetii e110q mutant 2- methylisocitrate lyase

Structure:

2-methylisocitrate lyase. Chain: a, b. Synonym: 2-mic,micl,(2r,3s)-2-methylisocitrate lyase. Engineered: yes. Mutation: yes

Source:

Coxiella burnetii. Organism_taxid: 777. Gene: prpb, cbu_0771. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.82Å R-factor: 0.236 R-free: 0.299

Authors:

W.Stuart,M.Isupov,N.J.Harmer

Key ref:

W.S.Stuart et al. (2025). Structure and catalytic mechanism of methylisocitrate a potential drug target against coxiella burnetii.. J.Biol.Chem., 301, 08517. PubMed id: 40250561 DOI: 10.1016/J.JBC.2025.108517

Date:

20-Nov-24 Release date: 13-Aug-25

Protein chains

Q83DG5  (Q83DG5_COXBU) -  2-methylisocitrate lyase from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)

Seq: 290 a.a.

Struc: 298 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.3.30 - methylisocitrate lyase.
• Reaction: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate (Bound ligand (Het Group name = EDO) matches with 66.67% similarity) + succinate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site