PDBsum entry 9fpy

Transcription

PDB id: 9fpy

Contents

Protein chains

304 a.a.

184 a.a.

155 a.a.

61 a.a.

124 a.a.

1268 a.a.

1129 a.a.

111 a.a.

671 a.a.

177 a.a.

545 a.a.

DNA/RNA

Metals

_MG ×4

_ZN ×4

Waters ×9

PDB id:

9fpy

Name:

Transcription

Title:

Structure of the complete vaccinia DNA-dependent RNA polymerase complex assembly intermediate 3

Structure:

DNA-directed RNA polymerase 35 kda subunit. Chain: c. Engineered: yes. DNA-directed RNA polymerase 22 kda subunit. Chain: e. Engineered: yes. DNA-directed RNA polymerase 18 kda subunit. Chain: g. Engineered: yes.

Source:

Orthopoxvirus vaccinia. Organism_taxid: 10245. Gene: opg156, rpo35, a29l. Expressed in: vaccinia virus. Expression_system_taxid: 10245. Gene: opg103, rpo22, j4r. Gene: opg119, rpo18, d7r. Gene: opg090, rpo7, g5.5r. Gene: opg073, e11l.

Authors:

C.Grimm,J.Bartuli,U.Fischer

Date:

14-Jun-24 Release date: 25-Jun-25

Protein chain

P21087  (RP35_VACCC) -  DNA-directed RNA polymerase 35 kDa subunit from Vaccinia virus (strain Copenhagen)

Seq: 305 a.a.

Struc: 304 a.a.*

Protein chain

P68608  (RP22_VACCC) -  DNA-directed RNA polymerase 22 kDa subunit from Vaccinia virus (strain Copenhagen)

Seq: 185 a.a.

Struc: 184 a.a.

Protein chain

P21034  (RP18_VACCC) -  DNA-directed RNA polymerase 18 kDa subunit from Vaccinia virus (strain Copenhagen)

Seq: 161 a.a.

Struc: 155 a.a.

Protein chain

P68315  (RP07_VACCC) -  DNA-directed RNA polymerase 7 kDa subunit from Vaccinia virus (strain Copenhagen)

Seq: 63 a.a.

Struc: 61 a.a.

Protein chains

P68449  (E11_VACCC) -  Core protein OPG073 from Vaccinia virus (strain Copenhagen)

Seq: 129 a.a.

Struc: 124 a.a.

Protein chain

P20504  (RP147_VACCC) -  DNA-directed RNA polymerase 147 kDa polypeptide from Vaccinia virus (strain Copenhagen)

Seq: 1286 a.a.

Struc: 1268 a.a.*

Protein chain

P68694  (RP132_VACCC) -  DNA-directed RNA polymerase 133 kDa polypeptide from Vaccinia virus (strain Copenhagen)

Seq: 1164 a.a.

Struc: 1129 a.a.*

Protein chain

Q76ZQ8  (RP19_VACCA) -  DNA-directed RNA polymerase 19 kDa subunit from Vaccinia virus (strain Ankara)

Seq: 164 a.a.

Struc: 111 a.a.*

Protein chain

P68439  (RAP94_VACCC) -  RNA polymerase-associated transcription-specificity factor RAP94 from Vaccinia virus (strain Copenhagen)

Seq: 795 a.a.

Struc: 671 a.a.

Protein chain

P21082  (RP30_VACCC) -  DNA-directed RNA polymerase 30 kDa polypeptide from Vaccinia virus (strain Copenhagen)

Seq: 259 a.a.

Struc: 177 a.a.

Protein chain

P20637  (NTP1_VACCC) -  Nucleoside triphosphatase I from Vaccinia virus (strain Copenhagen)

Seq: 631 a.a.

Struc: 545 a.a.*

* PDB and UniProt seqs differ at 12 residue positions (black crosses)

DNA/RNA chain

G-G-U-C-C-C-A-U-G-G-U-G-U-A-A-U-G-G-U-U-A-G-C-A-C-U-C-U-G-G-A-OMC-U-5MU-U-G-A- 72 bases

Enzyme reactions

• Enzyme class 2: Chains C, E, G, J, A, B, F, S: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: Chain Y: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site