PDBsum entry 9fjh

Cell cycle

PDB id: 9fjh

Contents

Protein chains

225 a.a.

35 a.a.

Ligands

PGE ×2

PG4

PO4

Waters ×52

PDB id:

9fjh

Name:

Cell cycle

Title:

Two plk1 pbd proteins bound to cenp-u(58-114) phosphorylated at thr78 and thr98

Structure:

Serine/threonine-protein kinase plk1. Chain: a, b. Synonym: polo-like kinase 1,plk-1,serine/threonine-protein kinase 13, stpk13. Engineered: yes. Centromere protein u. Chain: c. Synonym: cenp-u,centromere protein of 50 kda,cenp-50,interphase centromere complex protein 24,kshv latent nuclear antigen-interacting

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: plk1, plk. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: cenpu, icen24, klip1, mlf1ip, pbip1. Expression_system_taxid: 562

Resolution:

2.15Å R-factor: 0.218 R-free: 0.250

Authors:

L.Ren,R.Gasper,I.R.Vetter,A.Musacchio

Key ref:

L.Ren et al. Structural and molecular basis of kinaseregulated inner-Kinetochore recruitment of plk1 by cenp-U. To be published, .

Date:

31-May-24 Release date: 24-Sep-25

Protein chains

P53350  (PLK1_HUMAN) -  Serine/threonine-protein kinase PLK1 from Homo sapiens

Seq: 603 a.a.

Struc: 225 a.a.

Protein chain

Q71F23  (CENPU_HUMAN) -  Centromere protein U from Homo sapiens

Seq: 418 a.a.

Struc: 35 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.11.21 - polo kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site