PDBsum entry 9fid

Hydrolase

PDB id: 9fid

Contents

Protein chains

461 a.a.

71 a.a.

70 a.a.

72 a.a.

Metals

_NA ×3

_CA ×6

Waters ×106

PDB id:

9fid

Name:

Hydrolase

Title:

X-ray structure of furin (pcsk3) in complex with the pc1/3 (pcsk1) prodomain mutant r78k,r80a

Structure:

Furin. Chain: a, b, c. Synonym: dibasic-processing enzyme,paired basic amino acid residue- cleaving enzyme,pace. Engineered: yes. Neuroendocrine convertase 1. Chain: d, e, f. Synonym: nec 1,prohormone convertase 1,proprotein convertase 1,pc1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: furin, fur, pace, pcsk3. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293s. Gene: pcsk1, nec1. Expressed in: escherichia coli.

Resolution:

2.40Å R-factor: 0.228 R-free: 0.252

Authors:

S.O.Dahms,H.Brandstetter

Key ref:

R.Klaushofer et al. (2025). Structural insights into proprotein convertase activa facilitate the engineering of highly specific furin inhibitors.. Nat commun, 16, 8206. PubMed id: 40897699 DOI: 10.1038/S41467-025-63479-Y

Date:

29-May-24 Release date: 10-Sep-25

Protein chains

P09958  (FURIN_HUMAN) -  Furin from Homo sapiens

Seq: 794 a.a.

Struc: 461 a.a.

Protein chain

P29120  (NEC1_HUMAN) -  Neuroendocrine convertase 1 from Homo sapiens

Seq: 753 a.a.

Struc: 71 a.a.

Protein chain

P29120  (NEC1_HUMAN) -  Neuroendocrine convertase 1 from Homo sapiens

Seq: 753 a.a.

Struc: 70 a.a.

Protein chain

P29120  (NEC1_HUMAN) -  Neuroendocrine convertase 1 from Homo sapiens

Seq: 753 a.a.

Struc: 72 a.a.

Enzyme reactions

• Enzyme class 2: Chains A, B, C: E.C.3.4.21.75 - furin.
• Reaction: Release of mature proteins from their proproteins by cleavage of Arg- Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.
• Enzyme class 3: Chain D: E.C.3.4.21.93 - proprotein convertase 1.
• Reaction: Release of protein hormones, neuropeptides and renin from their precursors, generally by cleavage of -Lys-Arg-|- bonds.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.