PDBsum entry 9e3c

Transferase

PDB id: 9e3c

Contents

Protein chain

620 a.a.

Ligands

GLY-ARG-GLY-ARG-GLY-ARG

SFG

PDB id:

9e3c

Name:

Transferase

Title:

Cryo-em structure of prmt5/wdr77 in complex with 6s complex (grg local refine)

Structure:

Protein arginine n-methyltransferase 5. Chain: j. Synonym: 72 kda icln-binding protein,histone-arginine n- methyltransferase prmt5,jak-binding protein 1,shk1 kinase-binding protein 1 homolog,skb1hs. Engineered: yes. Small nuclear ribonucleoprotein sm d1. Chain: m. Synonym: sm-d1,sm-d autoantigen,snrnp core protein d1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: prmt5, hrmt1l5, ibp72, jbp1, skb1. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: snrpd1. Expressed in: escherichia coli. Expression_system_taxid: 562

Authors:

C.Y.Jin,M.Hunkeler,E.S.Fischer

Key ref:

C.Y.Jin et al. (2025). Substrate adaptors are flexible tethering modules tha enhance substrate methylation by the arginine methyltransferase prmt5.. J.Biol.Chem., 301, 08165. PubMed id: 39793893 DOI: 10.1016/J.JBC.2025.108165

Date:

23-Oct-24 Release date: 19-Feb-25

Protein chain

O14744  (ANM5_HUMAN) -  Protein arginine N-methyltransferase 5 from Homo sapiens

Seq: 637 a.a.

Struc: 620 a.a.

Enzyme reactions

• Enzyme class: E.C.2.1.1.320 - type Ii protein arginine methyltransferase.
• Reaction: L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L- homocysteine + 2 H⁺

L-arginyl-[protein] + 2 × S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 × S-adenosyl-L- homocysteine (Bound ligand (Het Group name = SFG) matches with 51.43% similarity) + 2 × H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site