PDBsum entry 9cgi

Transferase, viral protein

PDB id

9cgi

Loading ...

Contents

			Protein chains

					1314 a.a.


					101 a.a.


					201 a.a.


					117 a.a.

PDB id:

9cgi

Links
- PDBe
- RCSB
- MMDB
- JenaLib
- Proteopedia
- CATH
- SCOP
- PDBSWS
- ProSAT

Name:

Transferase, viral protein

Title:

Cryo-em structure of the nipah virus polymerase (l) protein in complex with the tetrameric phosphoprotein (p)

Structure:

RNA-directed RNA polymerase l. Chain: a. Synonym: protein l,large structural protein,replicase,transcriptase. Ec: 2.7.7.48,3.6.1.-,2.7.7.88,2.1.1.375. Engineered: yes. Phosphoprotein. Chain: b, c, d, e. Synonym: protein p. Engineered: yes

Source:

Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: p/v/c.

Authors:

B.Liu,G.Yang,D.Wang

Key ref:

G.Yang et al. Structure of the nipah virus polymerase phosphoprotei complex.. Nat commun, . PubMed id: 39375338

Date:

29-Jun-24

Release date:

18-Sep-24

PROCHECK

	Headers

	References

Protein chain

Q997F0 (L_NIPAV) - RNA-directed RNA polymerase L from Nipah virus

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					2244 a.a. 1314 a.a.

Protein chains

Q9IK91 (PHOSP_NIPAV) - Phosphoprotein from Nipah virus

Seq: Struc:

Seq: Struc:					709 a.a. 101 a.a.

Protein chain

Q9IK91 (PHOSP_NIPAV) - Phosphoprotein from Nipah virus

Seq: Struc:

Seq: Struc:					709 a.a. 201 a.a.

Protein chain

Q9IK91 (PHOSP_NIPAV) - Phosphoprotein from Nipah virus

Seq: Struc:

Seq: Struc:					709 a.a. 117 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class 2:

Chain A: E.C.2.1.1.375 - Nns virus cap methyltransferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl- adenosine in mRNA + 2 S-adenosyl-L-homocysteine + H⁺

5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA	+	2 × S-adenosyl-L-methionine	=	5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl- adenosine in mRNA	+	2 × S-adenosyl-L-homocysteine	+	H(+)

Enzyme class 3:

Chain A: E.C.2.7.7.48 - RNA-directed Rna polymerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

RNA(n)	+	2 × ribonucleoside 5'-triphosphate	=	RNA(n+1)	+	2 × diphosphate

Enzyme class 4:

Chain A: E.C.2.7.7.88 - Gdp polyribonucleotidyltransferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

a 5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + GDP + H⁺ = a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl- adenosine in mRNA + diphosphate

5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in mRNA	+	2 × GDP	+	H(+)	=	2 × 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl- adenosine in mRNA	+	diphosphate

Enzyme class 5:

Chain A: E.C.3.6.1.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site