PDBsum entry 9bc5

Viral protein/DNA

PDB id: 9bc5

Contents

Protein chains

(+ 0 more) 478 a.a.

277 a.a.

DNA/RNA

PDB id:

9bc5

Name:

Viral protein/DNA

Title:

Aav-2 rep68-aavs1 heptameric complex

Structure:

Protein rep68. Chain: a, b, c, d, e, f, g. Engineered: yes. Mutation: yes. Aavs1 DNA (41-mer) sense strand. Chain: h. Engineered: yes. Aavs1 DNA (41-mer) antisense. Chain: i.

Source:

Adeno-associated virus 2. Organism_taxid: 10804. Gene: rep68. Expressed in: synthetic construct. Expression_system_taxid: 32630. Synthetic: yes. Homo sapiens. Organism_taxid: 9606. Organism_taxid: 9606

Authors:

R.Jaiswal,C.R.Escalante

Key ref:

R.Jaiswal et al. (2025). Cryo-Em structure of aav2 rep68 bound to integration aavs1: insights into the mechanism of DNA melting.. Nucleic acids res., 53, . PubMed id: 39883011 DOI: 10.1093/NAR/GKAF033

Date:

07-Apr-24 Release date: 12-Feb-25

Protein chains

P03132  (REP68_AAV2S) -  Protein Rep68 from Adeno-associated virus 2 (isolate Srivastava/1982)

Seq: 536 a.a.

Struc: 478 a.a.*

Protein chain

P03132  (REP68_AAV2S) -  Protein Rep68 from Adeno-associated virus 2 (isolate Srivastava/1982)

Seq: 536 a.a.

Struc: 277 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

T-G-G-C-G-G-C-G-G-T-T-G-G-G-G-C-T-C-G-G-C-G-C-T-C-G-C-T-C-G-C-T-C-G-C-T-G-G-G- 41 bases

C-G-C-C-C-A-G-C-G-A-G-C-G-A-G-C-G-A-G-C-G-C-C-G-A-G-C-C-C-C-A-A-C-C-G-C-C-G-C- 41 bases

Enzyme reactions

• Enzyme class: Chains A, C, D, E, F, G: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site