PDBsum entry 9azh

Hydrolase

PDB id: 9azh

Contents

Protein chains

(+ 0 more) 369 a.a.

Ligands

GOL ×4

Metals

_NA ×3

_CL ×2

Waters ×1733

PDB id:

9azh

Name:

Hydrolase

Title:

Native nnha in p1

Structure:

2-nitroimidazole nitrohydrolase. Chain: a, b, c, d, e, f. Synonym: 2ni nitrohydrolase. Engineered: yes. Mutation: yes

Source:

Mycobacterium sp. Js330. Organism_taxid: 1004011. Gene: nnha. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693

Resolution:

2.04Å R-factor: 0.156 R-free: 0.179

Authors:

T.S.Peat,J.Newman

Key ref:

F.H.Ahmed et al. (2024). Structural insights into the enzymatic breakdown of azomycin-Derived antibiotics by 2-Nitroimdazole hydro (nnha).. Commun biol, 7, 1676. PubMed id: 39702827 DOI: 10.1038/S42003-024-07336-6

Date:

11-Mar-24 Release date: 01-Jan-25

Protein chains

F4ZCI3  (NNHA_MYCS0) -  2-nitroimidazole nitrohydrolase from Mycobacterium sp. (strain JS330)

Seq: 379 a.a.

Struc: 369 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.99.9 - 2-nitroimidazole nitrohydrolase.
• Reaction: 2-nitroimidazole + H2O = 1,3-dihydro-2H-imidazol-2-one + nitrite + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site