PDBsum entry 9ar5

DNA binding protein/DNA/RNA

PDB id: 9ar5

Contents

Protein chain

1105 a.a.

DNA/RNA

PDB id:

9ar5

Name:

DNA binding protein/DNA/RNA

Title:

Structure of acidothermus cellulolyticus cas9 bound with methylated DNA

Structure:

Crispr-associated endonuclease, csn1 family. Chain: a. Engineered: yes. RNA (92-mer). Chain: b. Engineered: yes. DNA (25-mer). Chain: x. Engineered: yes.

Source:

Acidothermus cellulolyticus. Organism_taxid: 28049. Gene: acel_1951. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 28049

Authors:

A.Das,J.Rai,B.Wang,H.Li

Key ref:

M.O.Roth et al. Molecular basis for epigenetic-Sensitive editing by c. To be published, .

Date:

22-Feb-24 Release date: 26-Feb-25

Protein chain

A0LWB3  (A0LWB3_ACIC1) -  CRISPR-associated endonuclease, Csn1 family from Acidothermus cellulolyticus (strain ATCC 43068 / DSM 8971 / 11B)

Seq: 1138 a.a.

Struc: 1105 a.a.

DNA/RNA chains

G-C-A-A-G-A-U-C-C-U-G-G-U-A-U-G-C-U-G-G-G-G-A-G-C-C-U-G-A-A-A-A-G-G-C-U-A-C-C- 92 bases

A-G-C-T-T-G-G-T-G-T-A-T-A-C-C-A-G-G-A-T-C-T-T-G-C 25 bases

T-A-C-A-5MC-C-A-A-G-C-T 11 bases