PDBsum entry 8xwc

DNA/hydrolase

PDB id: 8xwc

Contents

Protein chain

316 a.a.

DNA/RNA

Ligands

PEG

GOL ×3

Metals

_NA

_MG

Waters ×434

PDB id:

8xwc

Name:

DNA/hydrolase

Title:

Crystal structure of human 8-oxoguanine glycosylase k249h mutant bound to the substrate 8-oxoguanine DNA at ph 8.0 under 277 k

Structure:

DNA (5'-d( Ap Gp Cp Gp Tp Cp Cp Ap (8Og) p Gp Tp Cp Tp Ap Cp C)-3'). Chain: c. Engineered: yes. DNA (5'-d(p Gp Gp Tp Ap Gp Ap Cp Cp Tp Gp Gp Ap Cp Gp C)- 3'). Chain: d. Engineered: yes. N-glycosylase/DNA lyase.

Source:

Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Human. Gene: ogg1, mmh, mutm, ogh1. Expression_system_taxid: 562

Resolution:

1.45Å R-factor: 0.155 R-free: 0.179

Authors:

M.Unno,M.Koga,N.Minowa,S.Komuro,Y.Tanaka

Key ref:

M.Unno et al. Active residue mutation of hogg1 provides a novel act mutant with an alternative reaction pathway. To be published, . PubMed id: 40754315

Date:

16-Jan-24 Release date: 23-Jul-25

Protein chain

O15527  (OGG1_HUMAN) -  N-glycosylase/DNA lyase from Homo sapiens

Seq: 345 a.a.

Struc: 316 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

A-G-C-G-T-C-C-A-8OG-G-T-C-T-A-C-C 16 bases

G-G-T-A-G-A-C-C-T-G-G-A-C-G-C 15 bases

Enzyme reactions

• Enzyme class 1: E.C.3.2.2.- - ?????
• Enzyme class 2: E.C.4.2.99.18 - DNA-(apurinic or apyrimidinic site) lyase.
• Reaction: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)- 2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho- 2'-deoxyribonucleoside-DNA + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.