PDBsum entry 8xvq

Hydrolase

PDB id: 8xvq

Contents

Protein chains

520 a.a.

Ligands

FRU ×2

Metals

_CA ×4

Waters ×335

PDB id:

8xvq

Name:

Hydrolase

Title:

Crystal structure of inulosucrase from lactobacillus reuteri 121 in complex with fructose

Structure:

Inulosucrase. Chain: a, b. Synonym: levansucrase. Engineered: yes

Source:

Limosilactobacillus reuteri. Organism_taxid: 1598. Gene: inu, dph67_05265. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768

Resolution:

2.14Å R-factor: 0.180 R-free: 0.227

Authors:

D.Ni,X.Hou,M.Cheng,W.Xu,Y.Rao,W.Mu

Key ref:

D.Ni et al. Structure-Guided tunnel engineering to reveal the mol basis of sugar chain extension of inulosucrase. To be published, .

Date:

15-Jan-24 Release date: 22-Jan-25

Protein chains

Q8GP32  (Q8GP32_LIMRT) -  Inulosucrase from Limosilactobacillus reuteri

Seq: 798 a.a.

Struc: 520 a.a.

Enzyme reactions

• Enzyme class: E.C.2.4.1.9 - inulosucrase.
• Reaction: [(2->1)-beta-D-fructosyl](n) + sucrose = [(2->1)-beta-D-fructosyl](n+1) + D-glucose

[(2->1)-beta-D-fructosyl](n) + sucrose = [(2->1)-beta-D-fructosyl](n+1) + D-glucose (Bound ligand (Het Group name = FRU) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site