PDBsum entry 8vso

Peptide binding protein

PDB id: 8vso

Contents

Protein chain

234 a.a.

Ligands

ASP-ARG-SER-SER-SEP-ALA-PRO-ASN-VAL

WQN

Metals

_CL

_MG ×2

Waters ×217

PDB id:

8vso

Name:

Peptide binding protein

Title:

Ternary structure of 14-3-3 sigma, braf phosphopeptide (ps365) and compound 78 (1124378)

Structure:

14-3-3 protein sigma. Chain: b. Fragment: residues 1-231. Synonym: epithelial cell marker protein 1,stratifin. Engineered: yes. Serine/threonine-protein kinase b-raf phosphopeptide. Chain: p. Fragment: residues 361-369 (uniprot numbering). Synonym: proto-oncogene b-raf,p94,v-raf murine sarcoma viral oncogene

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: sfn, hme1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.50Å R-factor: 0.168 R-free: 0.189

Authors:

H.R.Vickery,J.M.Virta,M.Pennings,M.Konstantinidou,M.Van Den Oetelaar, R.J.Neitz,C.Ottmann,L.Brunsveld,M.R.Arkin

Key ref:

H.R.Vickery et al. Ternary structure of 14-3-3 sigma, Braf phosphopeptid (ps365) and compound 78 (1124378). To be published, .

Date:

24-Jan-24 Release date: 19-Mar-25

Protein chain

P31947  (1433S_HUMAN) -  14-3-3 protein sigma from Homo sapiens

Seq: 248 a.a.

Struc: 234 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site