PDBsum entry 8vqh

Oxidoreductase

PDB id: 8vqh

Contents

Protein chains

404 a.a.

419 a.a.

Ligands

SF4 ×2

Metals

_CU ×2

Waters ×2

PDB id:

8vqh

Name:

Oxidoreductase

Title:

Cryoem structure of bchn-bchb electron acceptor component protein of dpor

Structure:

Light-independent protochlorophyllide reductase subunit n. Chain: a, c. Synonym: dpor subunit n,li-por subunit n. Engineered: yes. Light-independent protochlorophyllide reductase subunit b. Chain: b, d. Synonym: dpor subunit b,li-por subunit b. Engineered: yes

Source:

Cereibacter sphaeroides. Organism_taxid: 1063. Gene: bchn, rskd131_1611. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: suf++ strain (pk11466). Gene: smt3, ydr510w, d9719.15, bchb, rhos4_18910, rsp_0286. Expression_system_variant: suf++ strain (pk11466)

Authors:

R.Kashyap,E.Antony

Key ref:

R.Kashyap and e.antony Cryoem structure of bchn-Bchb electron acceptor compo protein of dpor. To be published, . PubMed id: 40274796

Date:

18-Jan-24 Release date: 30-Apr-25

Protein chains

B9KK24  (BCHN_CERSK) -  Light-independent protochlorophyllide reductase subunit N from Cereibacter sphaeroides (strain KD131 / KCTC 12085)

Seq: 428 a.a.

Struc: 404 a.a.

Protein chains

Q12306  (SMT3_YEAST) -  Ubiquitin-like protein SMT3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 101 a.a.

Struc: 419 a.a.*

Protein chains

Q9Z5D9  (BCHB_RHOS4) -  Light-independent protochlorophyllide reductase subunit B from Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.)

Seq: 534 a.a.

Struc: 419 a.a.

* PDB and UniProt seqs differ at 92 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.1.3.7.7 - ferredoxin:protochlorophyllide reductase (ATP-dependent).
• Reaction: chlorophyllide a + oxidized 2[4Fe-4S]-[ferredoxin] + 2 ADP + 2 phosphate = protochlorophyllide a + reduced 2[4Fe-4S]-[ferredoxin] + 2 ATP + 2 H2O
• Cofactor: Iron-sulfur

Molecule diagrams generated from .mol files obtained from the KEGG ftp site