PDBsum entry 8vmf

Transferase/signaling protein

PDB id: 8vmf

Contents

Protein chains

355 a.a.

19 a.a.

Ligands

ALA-PRO-ASP-LEU-SER

AF3

ADP

GOL ×3

Metals

_CL

_MG ×2

Waters ×131

PDB id:

8vmf

Name:

Transferase/signaling protein

Title:

Crystal structure of a transition-state mimic of the gsk-3/axin complex bound to a beta-catenin s45d peptide

Structure:

Glycogen synthase kinase-3 beta. Chain: a. Synonym: gsk-3 beta,serine/threonine-protein kinase gsk3b. Engineered: yes. Axin-1. Chain: b. Synonym: axis inhibition protein 1,protein fused. Engineered: yes. Catenin beta-1.

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Gene: gsk3b. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Homo sapiens. Human. Organism_taxid: 9606.

Resolution:

2.50Å R-factor: 0.210 R-free: 0.251

Authors:

M.D.Enos,M.Gavagan,N.Jameson,J.G.Zalatan,W.I.Weis

Key ref:

M.D.Enos et al. Structural and functional effects of phosphopriming a scaffolding in the kinase gsk3beta. Sci signal, . PubMed id: 39226374

Date:

13-Jan-24 Release date: 28-Aug-24

Protein chain

Q9WV60  (GSK3B_MOUSE) -  Glycogen synthase kinase-3 beta from Mus musculus

Seq: 420 a.a.

Struc: 355 a.a.*

Protein chain

O15169  (AXIN1_HUMAN) -  Axin-1 from Homo sapiens

Seq: 862 a.a.

Struc: 19 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + H(+)

• Enzyme class 2: Chain A: E.C.2.7.11.26 - [tau protein] kinase.
• Reaction:
  1. L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H⁺
  2. L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H⁺

L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + H(+)

L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site