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PDBsum entry 8vmc
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Biosynthetic protein
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PDB id
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8vmc
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PDB id:
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| Name: |
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Biosynthetic protein
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Title:
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Composite structure of human fasn with NADPH in state 6
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Structure:
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Fatty acid synthase. Chain: a, b. Synonym: type i fatty acid synthase. Ec: 2.3.1.85,2.3.1.38,2.3.1.39,2.3.1.41,1.1.1.100,4.2.1.59,1.3.1.39, 3.1.2.14. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: fasn, fas. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9
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Authors:
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K.Schultz,R.Marmorstein
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Key ref:
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K.Schultz
et al.
Snapshots of acyl carrier protein shuttling in human acid synthase..
Nature,
.
PubMed id:
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Date:
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13-Jan-24
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Release date:
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26-Feb-25
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PROCHECK
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Headers
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References
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P49327
(FAS_HUMAN) -
Fatty acid synthase from Homo sapiens
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Seq:
Struc:
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2511 a.a.
2068 a.a.*
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Key: |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 1:
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E.C.1.1.1.100
- 3-oxoacyl-[acyl-carrier-protein] reductase.
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Reaction:
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a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + NADPH + H+
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(3R)-hydroxyacyl-[ACP]
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+
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NADP(+)
Bound ligand (Het Group name = )
corresponds exactly
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=
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3-oxoacyl-[ACP]
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+
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NADPH
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+
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H(+)
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Enzyme class 2:
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E.C.1.3.1.39
- enoyl-[acyl-carrier-protein] reductase (Nadph, Re-specific).
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Reaction:
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a 2,3-saturated acyl-[ACP] + NADP+ = a (2E)-enoyl-[ACP] + NADPH + H+
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2,3-saturated acyl-[ACP]
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+
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NADP(+)
Bound ligand (Het Group name = )
corresponds exactly
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=
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(2E)-enoyl-[ACP]
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+
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NADPH
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+
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H(+)
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Enzyme class 3:
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E.C.2.3.1.38
- [acyl-carrier-protein] S-acetyltransferase.
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Reaction:
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holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA
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holo-[ACP]
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+
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acetyl-CoA
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=
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acetyl-[ACP]
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+
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CoA
Bound ligand (Het Group name = )
matches with 52.38% similarity
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Enzyme class 4:
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E.C.2.3.1.39
- [acyl-carrier-protein] S-malonyltransferase.
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Reaction:
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holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA
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holo-[ACP]
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+
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malonyl-CoA
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=
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malonyl-[ACP]
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+
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CoA
Bound ligand (Het Group name = )
matches with 52.38% similarity
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Enzyme class 5:
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E.C.2.3.1.41
- beta-ketoacyl-[acyl-carrier-protein] synthase I.
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Reaction:
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a fatty acyl-[ACP] + malonyl-[ACP] + H+ = a 3-oxoacyl-[ACP] + holo- [ACP] + CO2
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fatty acyl-[ACP]
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+
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malonyl-[ACP]
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+
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H(+)
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=
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3-oxoacyl-[ACP]
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+
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holo- [ACP]
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+
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CO2
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Enzyme class 6:
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E.C.2.3.1.85
- fatty-acid synthase system.
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Reaction:
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acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+
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acetyl-CoA
Bound ligand (Het Group name = )
matches with 50.00% similarity
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+
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n
malonyl-CoA
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+
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2n NADPH
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+
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2n H(+)
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=
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long-chain fatty acid
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+
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(n+1) CoA
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+
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n
CO2
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+
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2n NADP(+)
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Enzyme class 7:
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E.C.3.1.2.14
- oleoyl-[acyl-carrier-protein] hydrolase.
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Reaction:
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(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H+
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Oleoyl-[acyl-carrier-protein]
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+
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n
H(2)O
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=
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[acyl-carrier-protein]
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+
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oleate
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Enzyme class 8:
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E.C.4.2.1.59
- 3-hydroxyacyl-[acyl-carrier-protein] dehydratase.
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Reaction:
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a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
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(3R)-3-hydroxyacyl-[acyl-carrier protein]
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=
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n
trans-2-enoyl-[acyl- carrier protein]
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+
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H(2)O
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
