PDBsum entry 8vbs

Transferase

PDB id: 8vbs

Contents

Protein chains

404 a.a.

132 a.a.

PDB id:

8vbs

Name:

Transferase

Title:

E. Coli cysteine desulfurase sufs bound to sufe c51a

Structure:

Cysteine desulfurase. Chain: a, b. Engineered: yes. Cysteine desulfuration protein sufe. Chain: c, d. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: sufs. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sufe. Expression_system_taxid: 562

Resolution:

3.31Å R-factor: 0.235 R-free: 0.272

Authors:

J.A.Dunkle,P.A.Frantom,N.C.Gilbert

Key ref:

R.Gogar et al. E. Coli cysteine desulfurase sufs bound to sufe c51a. To be published, . PubMed id: 39122000

Date:

12-Dec-23 Release date: 16-Oct-24

Protein chains

P77444  (SUFS_ECOLI) -  Cysteine desulfurase from Escherichia coli (strain K12)

Seq: 406 a.a.

Struc: 404 a.a.*

Protein chains

J7Q7S7  (J7Q7S7_ECOLX) -  Cysteine desulfuration protein SufE from Escherichia coli

Seq: 138 a.a.

Struc: 132 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.2.8.1.7 - cysteine desulfurase.
• Reaction: (sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine
• Cofactor: Pyridoxal 5'-phosphate
• Enzyme class 3: Chains A, B: E.C.3.13.1.- - ?????
• Enzyme class 4: Chains A, B: E.C.4.4.1.16 - selenocysteine lyase.
• Reaction: L-selenocysteine + AH2 = hydrogenselenide + L-alanine + A + H⁺
• Cofactor: Pyridoxal 5'-phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site