PDBsum entry 8v4l

Hydrolase/substrate,structural protein

PDB id: 8v4l

Contents

Protein chains

435 a.a.

435 a.a.

501 a.a.

Ligands

GTP ×2

G2P ×2

GLU

Metals

_ZN

_MG ×4

PDB id:

8v4l

Name:

Hydrolase/substrate,structural protein

Title:

Ccp5 in complex with microtubules class2

Structure:

Tubulin alpha-1b chain. Chain: a, c. Synonym: alpha-tubulin ubiquitous, tubulin k-alpha-1, tubulin alpha- ubiquitous chain. Tubulin beta chain. Chain: b, d. Synonym: beta-tubulin. Other_details: beta tubulin from porcine brain microtubules, heterogeneous in tail sequence.

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Organ: brain. Homo sapiens. Human. Organism_taxid: 9606. Gene: agbl5. Expressed in: spodoptera frugiperda.

Authors:

J.Chen,E.A.Zehr,J.M.Gruschus,A.Szyk,Y.Liu,M.E.Tanner,N.Tjandra, A.Roll-Mecak

Key ref:

J.Chen et al. Tubulin code eraser ccp5 binds branch glutamates by substrate deformation. Nature, . PubMed id: 39020174

Date:

29-Nov-23 Release date: 17-Jul-24

Protein chains

Q2XVP4  (TBA1B_PIG) -  Tubulin alpha-1B chain from Sus scrofa

Seq: 451 a.a.

Struc: 435 a.a.

Protein chains

P02554  (TBB_PIG) -  Tubulin beta chain from Sus scrofa

Seq: 445 a.a.

Struc: 435 a.a.*

Protein chain

Q8NDL9  (CBPC5_HUMAN) -  Cytosolic carboxypeptidase-like protein 5 from Homo sapiens

Seq: 886 a.a.

Struc: 501 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, C: E.C.3.6.5.- - ?????
• Enzyme class 2: Chain E: E.C.3.4.17.- - ?????
• Enzyme class 3: Chain E: E.C.3.4.17.24 - tubulin-glutamate carboxypeptidase.
• Reaction:
  1. C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-glutamate
  2. C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-[tubulin] + L-glutamate

C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-glutamate (Bound ligand (Het Group name = GLU) corresponds exactly)

C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-[tubulin] + L-glutamate (Bound ligand (Het Group name = GLU) corresponds exactly)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site