PDBsum entry 8v0e

Ligase

PDB id: 8v0e

Contents

Protein chains

344 a.a.

Ligands

UNK-UNK-UNK-UNK-UNK

Waters ×151

PDB id:

8v0e

Name:

Ligase

Title:

Ank repeat of mib1

Structure:

E3 ubiquitin-protein ligase mib1. Chain: a, b. Fragment: ank repeat. Engineered: yes. Unidentified mib1 peptide. Chain: d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mib1. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.39Å R-factor: 0.208 R-free: 0.244

Authors:

R.Cao,S.C.Blacklow

Key ref:

R.Cao et al. Structural requirements for activity of mind bomb1 in signaling.. Structure, . PubMed id: 39121852

Date:

17-Nov-23 Release date: 25-Sep-24

Protein chains

Q86YT6  (MIB1_HUMAN) -  E3 ubiquitin-protein ligase MIB1 from Homo sapiens

Seq: 1006 a.a.

Struc: 344 a.a.

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine