PDBsum entry 8uw3

RNA binding protein/RNA/DNA

PDB id: 8uw3

Contents

Protein chain

1265 a.a.

DNA/RNA

Ligands

TTP

PDB id:

8uw3

Name:

RNA binding protein/RNA/DNA

Title:

Human line-1 retrotransposon orf2 protein engaged with template RNA in elongation state

Structure:

Line-1 retrotransposable element orf2 protein. Chain: a. Engineered: yes. Template RNA. Chain: b. Engineered: yes. Complementary DNA. Chain: c. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Authors:

A.Thawani,A.J.Florez Ariza,K.Collins,E.Nogales

Key ref:

A.Thawani et al. Template and target site recognition by human line-1 retrotransposition. Nature, . PubMed id: 38096901

Date:

06-Nov-23 Release date: 20-Dec-23

Protein chain

O00370  (LORF2_HUMAN) -  LINE-1 retrotransposable element ORF2 protein from Homo sapiens

Seq: 1275 a.a.

Struc: 1265 a.a.*

* PDB and UniProt seqs differ at 16 residue positions (black crosses)

DNA/RNA chains

G-C-A-G-A-C-U-G-C-G-U-A-G-G-C-G-U-G-C-G-C-A-C-G-C-C-U-A-C-G-C-A-G-U-C-U-G-C-A- 63 bases

G-C-C-G-A-T-A-T-T-DDG 10 bases

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 2: E.C.3.1.21.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site