PDBsum entry 8u72

RNA binding protein/RNA/DNA

PDB id: 8u72

Contents

Protein chains

417 a.a.

454 a.a.

DNA/RNA

Metals

_MG ×4

Waters ×57

PDB id:

8u72

Name:

RNA binding protein/RNA/DNA

Title:

Cryo-em structure of the sparta oligomer with guide RNA and target dna

Structure:

RNA (5'- r(p Up Gp Ap Gp Gp Up Ap Gp Up Ap Gp Gp Up Up Gp Up Ap Up A)-3'). Chain: u, m, s, x. Engineered: yes. DNA (5'- d(p Tp Ap Tp Ap Cp Ap Ap Cp Cp Tp Ap Cp Tp Ap Cp Cp Tp C)-3'). Chain: v, n, t, y. Engineered: yes. Tir domain-containing protein.

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Thermoflavifilum thermophilum. Organism_taxid: 1393122. Gene: samn05660895_1670. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: samn05660895_1671.

Authors:

R.Malik,J.Kottur,A.K.Aggarwal

Key ref:

J.Kottur et al. Nucleic acid mediated activation of prokaryotic argon immune system. Nat commun, . PubMed id: 38844755

Date:

14-Sep-23 Release date: 12-Jun-24

Protein chains

A0A1I7NFG5  (A0A1I7NFG5_9BACT) -  TIR domain-containing protein from Thermoflavifilum thermophilum

Seq: 450 a.a.

Struc: 417 a.a.

Protein chains

A0A1I7NFD7  (A0A1I7NFD7_9BACT) -  Piwi domain-containing protein from Thermoflavifilum thermophilum

Seq: 507 a.a.

Struc: 454 a.a.

DNA/RNA chains

U-G-A-G-G-U-A-G-U-A-G-G-U-U-G-U-A-U-A 19 bases

T-A-T-A-C-A-A-C-C-T-A-C-T-A-C-C-T-C 18 bases

U-G-A-G-G-U-A-G-U-A-G-G-U-U-G-U-A-U-A 19 bases

T-A-T-A-C-A-A-C-C-T-A-C-T-A-C-C-T-C 18 bases

U-G-A-G-G-U-A-G-U-A-G-G-U-U-G-U-A-U-A 19 bases

T-A-T-A-C-A-A-C-C-T-A-C-T-A-C-C-T-C 18 bases

U-G-A-G-G-U-A-G-U-A-G-G-U-U-G-U-A-U-A 19 bases

T-A-T-A-C-A-A-C-C-T-A-C-T-A-C-C-T-C 18 bases