PDBsum entry 8t6t

Immune system

PDB id: 8t6t

Contents

Protein chain

1168 a.a.

DNA/RNA

PDB id:

8t6t

Name:

Immune system

Title:

Spry-cas9:grna complex targeting tac pam DNA with 13 bp r-loop

Structure:

Crispr-associated endonuclease cas9/csn1. Chain: a. Synonym: spcas9,spycas9. Engineered: yes. Mutation: yes. Grna. Chain: b. Engineered: yes. Ts.

Source:

Streptococcus pyogenes. Organism_taxid: 1314. Gene: cas9, csn1, spy_1046. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Escherichia phage lambda. Organism_taxid: 2681611. Organism_taxid: 2681611

Authors:

G.N.Hibshman,J.P.K.Bravo,D.W.Taylor

Key ref:

G.N.Hibshman et al. Unraveling the mechanisms of pamless DNA interrogatio spry-Cas9. Nat commun, . PubMed id: 38688943

Date:

18-Jun-23 Release date: 01-May-24

Protein chain

Q99ZW2  (CAS9_STRP1) -  CRISPR-associated endonuclease Cas9/Csn1 from Streptococcus pyogenes serotype M1

Seq: 1368 a.a.

Struc: 1168 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

DNA/RNA chains

G-C-A-U-A-A-A-G-A-U-G-A-G-A-C-G-C-G-U-U-U-U-A-G-A-G-C-U-A-G-A-A-A-U-A-G-C-A-A- 95 bases

T-T-G-T-A-C-T-G-T-A-G-C-G-T-C-T-C-A-T-C-T-T-T-A 24 bases

T-A-C-A-G-T-A-C-A-A 10 bases

Enzyme reactions

• Enzyme class: E.C.3.1.-.-