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PDBsum entry 8sxw

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protein metals Protein-protein interface(s) links
Isomerase PDB id
8sxw

 

 

 

 

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Contents
Protein chains
361 a.a.
Metals
_NA
_CL ×2
Waters ×1111
PDB id:
8sxw
Name: Isomerase
Title: X-ray crystal structure of udp- 2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from thermus thermophilus strain hb27, d98n mutation, apo structure at ph 6
Structure: Udp-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2- epimerase. Chain: a, b, c, d. Engineered: yes. Mutation: yes
Source: Thermus thermophilus hb27. Organism_taxid: 262724. Strain: atcc baa-163 / dsm 7039 / hb27. Gene: tt_c0285. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta2
Resolution:
1.80Å     R-factor:   0.181     R-free:   0.224
Authors: C.W.Kroft,J.B.Thoden,H.M.Holden
Key ref: J.B.Thoden et al. Structural analysis of a bacterial udp-Sugar 2-Epimer reveals the active site architecture before and after catalysis.. J biol chem, . PubMed id: 37660908
Date:
24-May-23     Release date:   13-Sep-23    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q72KY0  (Q72KY0_THET2) -  UDP-N-acetylglucosamine 2-epimerase from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)
Seq:
Struc:
365 a.a.
361 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.5.1.3.14  - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
UDP-N-acetylgalactosamine and UDP-N-acetylmannosamine Biosynthesis
      Reaction: UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
UDP-N-acetyl-D-glucosamine
= UDP-N-acetyl-D-mannosamine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 

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