PDBsum entry 8swc

Hydrolase/RNA

PDB id: 8swc

Contents

Protein chain

149 a.a.

DNA/RNA

Ligands

PO4 ×2

SO4

Metals

_CA ×3

Waters ×5

PDB id:

8swc

Name:

Hydrolase/RNA

Title:

Rnase h complex with aso (ooo) and RNA

Structure:

Ribonuclease h1. Chain: a. Synonym: rnase h1,ribonuclease h type ii. Engineered: yes. RNA (5'- r( Up Gp Gp Cp Gp Ap Gp Up Gp Gp Gp Up Gp Ap Gp Up Gp Ap Gp G)-3'). Chain: b. Engineered: yes. Po aso.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rnaseh1, rnh1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.68Å R-factor: 0.193 R-free: 0.220

Authors:

Y.-J.Cho,N.Iwamoto

Key ref:

N.Iwamoto and y.-j.cho Crystal structure of rnase h/rna/po-Aso complex at an level. To be published, .

Date:

18-May-23 Release date: 19-Jun-24

Protein chain

O60930  (RNH1_HUMAN) -  Ribonuclease H1 from Homo sapiens

Seq: 286 a.a.

Struc: 149 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

U-G-G-C-G-A-G-U-G-G-G-U-G-A-G-U-G-A-G-G 20 bases

OMC-C5L-T39-C5L-A2M-C-T-C-A-C-C-C-A-C-T-OMC-OMG-OMC-OMC-A2M 20 bases

Enzyme reactions

• Enzyme class: E.C.3.1.26.4 - ribonuclease H.
• Reaction: Endonucleolytic cleavage to 5'-phosphomonoester.