PDBsum entry 8ssl

Isomerase

PDB id: 8ssl

Contents

Protein chains

1072 a.a.

Ligands

GDP ×3

Metals

_MG

PDB id:

8ssl

Name:

Isomerase

Title:

Isobutyryl-coa mutase fused q341a in the presence of gtp

Structure:

Fused isobutyryl-coa mutase. Chain: a, b, c. Engineered: yes. Mutation: yes

Source:

Cupriavidus metallidurans ch34. Organism_taxid: 266264. Gene: icmf, sbm, rmet_0210. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Authors:

F.A.Vaccaro,C.L.Drennan

Key ref:

F.A.Vaccaro et al. (2023). Structural insight into g-Protein chaperone-Mediated maturation of a bacterial adenosylcobalamin-Dependent mutase.. J.Biol.Chem., 299, 05109. PubMed id: 37517695 DOI: 10.1016/J.JBC.2023.105109

Date:

08-May-23 Release date: 08-May-24

Protein chains

Q1LRY0  (ICMF_CUPMC) -  Fused isobutyryl-CoA mutase from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

Seq: 1093 a.a.

Struc: 1072 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.3.6.5.- - ?????
• Enzyme class 3: E.C.5.4.99.13 - isobutyryl-CoA mutase.
• Reaction: 2-methylpropanoyl-CoA = butanoyl-CoA
• Cofactor: Cobalt cation

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site