PDBsum entry 8ssj

Transferase

PDB id: 8ssj

Contents

Protein chains

455 a.a.

Metals

_CL ×2

Waters ×260

PDB id:

8ssj

Name:

Transferase

Title:

Room-temperature x-ray structure of human mitochondrial serine hydroxymethyltransferase (hshmt2)

Structure:

Serine hydroxymethyltransferase, mitochondrial. Chain: a, b. Synonym: shmt,glycine hydroxymethyltransferase,serine methylase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: shmt2. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.50Å R-factor: 0.174 R-free: 0.205

Authors:

V.N.Drago,A.Kovalevsky

Key ref:

V.N.Drago et al. (2023). Revealing protonation states and tracking substrate i serine hydroxymethyltransferase with room-Temperature and neutron crystallography.. Commun chem, 6, 162. PubMed id: 37532884

Date:

08-May-23 Release date: 16-Aug-23

Protein chains

P34897  (GLYM_HUMAN) -  Serine hydroxymethyltransferase, mitochondrial from Homo sapiens

Seq: 504 a.a.

Struc: 455 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.1.2.1 - glycine hydroxymethyltransferase.
• Pathway: Folate Coenzymes
• Reaction: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)- 5,6,7,8-tetrahydrofolate + L-serine
• Cofactor: Pyridoxal 5'-phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site