PDBsum entry 8sqa

Membrane protein

PDB id: 8sqa

Contents

Protein chains

765 a.a.

369 a.a.

61 a.a.

215 a.a.

85 a.a.

68 a.a.

PDB id:

8sqa

Name:

Membrane protein

Title:

The cryo-em structure of the ecbam/espp(beta8-12) complex

Structure:

Outer membrane protein assembly factor bama. Chain: a. Engineered: yes. Outer membrane protein assembly factor bamb. Chain: b. Engineered: yes. Outer membrane protein assembly factor bamc. Chain: c. Engineered: yes.

Source:

Escherichia coli. Organism_taxid: 562. Gene: bama. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: bamb, yfgl, b2512, jw2496. Gene: bamc. Gene: bamd. Gene: bame, smpa, smpa_1, smpa_2.

Authors:

R.Wu,N.Noinaj

Key ref:

R.Wu et al. Bam orchestrates omp biogenesis using a beta-Templati mechanism. To be published, .

Date:

04-May-23 Release date: 08-May-24

Protein chain

P0A940  (BAMA_ECOLI) -  Outer membrane protein assembly factor BamA from Escherichia coli (strain K12)

Seq: 810 a.a.

Struc: 765 a.a.*

Protein chain

P77774  (BAMB_ECOLI) -  Outer membrane protein assembly factor BamB from Escherichia coli (strain K12)

Seq: 392 a.a.

Struc: 369 a.a.

Protein chain

P0A903  (BAMC_ECOLI) -  Outer membrane protein assembly factor BamC from Escherichia coli (strain K12)

Seq: 344 a.a.

Struc: 61 a.a.

Protein chain

P0AC02  (BAMD_ECOLI) -  Outer membrane protein assembly factor BamD from Escherichia coli (strain K12)

Seq: 245 a.a.

Struc: 215 a.a.

Protein chain

P0A937  (BAME_ECOLI) -  Outer membrane protein assembly factor BamE from Escherichia coli (strain K12)

Seq: 113 a.a.

Struc: 85 a.a.

Protein chain

O32591  (ESPP_ECOLX) -  Serine protease EspP from Escherichia coli

Seq: 1300 a.a.

Struc: 68 a.a.*

Protein chain

P0AEX9  (MALE_ECOLI) -  Maltose/maltodextrin-binding periplasmic protein from Escherichia coli (strain K12)

Seq: 396 a.a.

Struc: 68 a.a.*

* PDB and UniProt seqs differ at 55 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain F: E.C.3.4.21.- - ?????