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PDBsum entry 8spk
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PDB id:
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Hydrolase
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Title:
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Crystal structure of antarctic pet-degrading enzyme
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Structure:
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Lipase 1. Chain: a, b. Synonym: triacylglycerol lipase. Engineered: yes
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Source:
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Moraxella. Organism_taxid: 475. Gene: lip1, l1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Resolution:
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1.60Å
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R-factor:
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0.154
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R-free:
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0.180
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Authors:
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A.A.Furtado,P.Blazquez-Sanchez,A.Grinen,J.A.Vargas,D.A.Leonardo, S.A.Sculaccio,H.M.Pereira,B.Diez,R.C.Garratt,C.A.Ramirez-Sarmiento
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Key ref:
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P.Blazquez-Sanchez
et al.
Engineering the catalytic activity of an antarctic pet-Degrading enzyme by loop exchange..
Protein sci,
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PubMed id:
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Date:
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03-May-23
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Release date:
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23-Aug-23
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PROCHECK
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Headers
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References
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P19833
(LIP1_MORS1) -
Lipase 1 from Moraxella sp. (strain TA144)
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Seq:
Struc:
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319 a.a.
265 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 4 residue positions (black
crosses)
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Enzyme class:
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E.C.3.1.1.3
- triacylglycerol lipase.
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Reaction:
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a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H+
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triacylglycerol
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+
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H2O
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=
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diacylglycerol
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+
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fatty acid
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+
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H(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
