PDBsum entry 8sko

Hydrolase/inhibitor

PDB id: 8sko

Contents

Protein chains

230 a.a.

Ligands

X8Z ×2

Metals

_ZN ×4

Waters ×526

PDB id:

8sko

Name:

Hydrolase/inhibitor

Title:

X-ray structure of the ndm-4 beta-lactamase from klebsiella pneumonia with l-captopril bound

Structure:

Metallo-beta-lactamase type 2. Chain: a, b. Synonym: b2 metallo-beta-lactamase,beta-lactamase type ii,metallo- beta-lactamase ndm-1,metallo-beta-lactamase type ii,new delhi metallo-beta-lactamase-1,ndm-1. Engineered: yes. Mutation: yes

Source:

Klebsiella pneumoniae. Organism_taxid: 573. Gene: blandm-1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta2

Resolution:

1.30Å R-factor: 0.200 R-free: 0.223

Authors:

J.B.Thoden,H.M.Holden

Key ref:

J.B.Thoden et al. Characterization of a novel inhibitor for the new del metallo-Beta-Lactamase-4: implications for drug desig combating bacterial drug resistance.. J biol chem, . PubMed id: 37549809

Date:

20-Apr-23 Release date: 23-Aug-23

Protein chains

C7C422  (BLAN1_KLEPN) -  Metallo-beta-lactamase type 2 from Klebsiella pneumoniae

Seq: 270 a.a.

Struc: 230 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)