PDBsum entry 8sij

Lyase

PDB id: 8sij

Contents

Protein chains

445 a.a.

Ligands

PLP

K9E

Metals

_CL ×2

Waters ×60

PDB id:

8sij

Name:

Lyase

Title:

Crystal structure of f. Varium tryptophanase

Structure:

Tryptophanase 1. Chain: a, b. Engineered: yes

Source:

Fusobacterium varium. Organism_taxid: 856. Gene: tnaa1. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.60Å R-factor: 0.241 R-free: 0.260

Authors:

A.L.Graboski,M.R.Redinbo

Key ref:

A.L.Graboski and m.r.redinbo Mechanism-Based inhibition of gut microbial tryptopha reduces serum indoxyl sulfate. Cell chem biol, . PubMed id: 37633277

Date:

16-Apr-23 Release date: 23-Aug-23

Protein chains

A0A448M3A5  (A0A448M3A5_FUSVA) -  Tryptophanase 1 from Fusobacterium varium

Seq: 463 a.a.

Struc: 445 a.a.

Enzyme reactions

• Enzyme class: E.C.4.1.99.1 - tryptophanase.
• Reaction: L-tryptophan + H2O = indole + pyruvate + NH4⁺

L-tryptophan + H2O (Bound ligand (Het Group name = K9E) matches with 40.74% similarity) = indole + pyruvate + NH4(+)

• Cofactor: K(+); Pyridoxal 5'-phosphate

K(+) Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site