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PDBsum entry 8sfd
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PDB id:
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Hydrolase
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Title:
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Crystal structure of the engineered ssopox variant ivb10
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Structure:
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Aryldialkylphosphatase. Chain: d. Synonym: paraoxonase,ssopox,phosphotriesterase-like lactonase. Engineered: yes
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Source:
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Saccharolobus solfataricus (strain atcc 35092 / dsm 1617 / jcm 11322 / p2). Organism_taxid: 273057. Strain: atcc 35092 / dsm 1617 / jcm 11322 / p2. Variant: ivb10. Gene: php, sso2522. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768
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Resolution:
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1.50Å
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R-factor:
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0.140
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R-free:
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0.173
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Authors:
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P.Jacquet,R.Billot,A.Shimon,N.Hoekstra,C.Bergonzi,A.Jenks,D.Daude, M.H.Elias
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Key ref:
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P.Jacquet
et al.
Changes in active site loops conformation relates to transition from lactonase to phosphotriesterase.
To be published,
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PubMed id:
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Date:
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10-Apr-23
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Release date:
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17-Apr-24
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PROCHECK
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Headers
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References
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Q97VT7
(PHP_SULSO) -
Aryldialkylphosphatase from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
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Seq:
Struc:
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314 a.a.
314 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 6 residue positions (black
crosses)
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Enzyme class:
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E.C.3.1.8.1
- aryldialkylphosphatase.
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Reaction:
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An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol
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aryl dialkyl phosphate
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+
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H2O
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=
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dialkyl phosphate
Bound ligand (Het Group name = )
matches with 71.43% similarity
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+
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aryl alcohol
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Cofactor:
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Divalent cation
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
