PDBsum entry 8sdr

Hydrolase/hydrolase inhibitor

PDB id: 8sdr

Contents

Protein chain

360 a.a.

Ligands

CB4

IMD

Waters ×593

PDB id:

8sdr

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of pdc-3 beta-lactamase in complex with the boronic acid inhibitor lp-06

Structure:

Beta-lactamase. Chain: a. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Gene: ampc. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.35Å R-factor: 0.131 R-free: 0.162

Authors:

V.Kumar,F.Van Den Akker

Key ref:

A.R.Mack et al. Natural protein engineering in the omega-Loop: the ro y221 in ceftazidime and ceftolozane resistance in pseudomonas-Derived cephalosporinase (pdc). To be published, . PubMed id: 37850746

Date:

07-Apr-23 Release date: 16-Aug-23

Protein chain

P24735  (AMPC_PSEAE) -  Beta-lactamase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 397 a.a.

Struc: 360 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)