PDBsum entry 8sdd

Hydrolase

PDB id: 8sdd

Contents

Protein chains

296 a.a.

Waters ×667

PDB id:

8sdd

Name:

Hydrolase

Title:

Crystal structure of fluoroacetate dehalogenase daro3835 h274n mutant with d107-glycolyl intermediate

Structure:

Alpha/beta hydrolase fold protein. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Dechloromonas aromatica rcb. Organism_taxid: 159087. Gene: daro_3835. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.221 R-free: 0.262

Authors:

P.J.Stogios,T.Skarina,A.Khusnutdinova,A.Iakounine,A.Savchenko

Key ref:

A.N.Khusnutdinova et al. (2023). Structural insights into hydrolytic defluorination of difluoroacetate by microbial fluoroacetate dehalogena. Febs j, 290, 4966. PubMed id: 37437000

Date:

06-Apr-23 Release date: 14-Feb-24

Protein chains

Q479B8  (Q479B8_DECAR) -  Alpha/beta hydrolase fold protein from Dechloromonas aromatica (strain RCB)

Seq: 303 a.a.

Struc: 296 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.?