PDBsum entry 8s3m

Viral protein

PDB id: 8s3m

Contents

Protein chain

345 a.a.

Ligands

FGA-API-LYS-ARG

PO4 ×2

Metals

_ZN

Waters ×65

PDB id:

8s3m

Name:

Viral protein

Title:

Lystt72, a lytic endopeptidase from thermus thermophilus mat72 phage vb_tt72

Structure:

Lytic endopeptidase. Chain: a. Engineered: yes. Gamma-d-glu-m-a2pm-l-lys-l-arg. Chain: c

Source:

Thermus phage tt72. Organism_taxid: 2978976. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: bl21(de3)[prare]. Escherichia coli bl21(de3). Organism_taxid: 469008

Resolution:

2.20Å R-factor: 0.187 R-free: 0.260

Authors:

W.Rypniewski,K.Biniek-Antosiak,M.Bejger

Key ref:

S.Dorawa et al. (2025). Crystal structure, Enzymatic and thermodynamic proper the thermus thermophilus phage tt72 lytic endopeptida unique structural signatures of thermal adaptation.. J.Struct.Biol., 217, 08230. PubMed id: 40580998 DOI: 10.1016/J.JSB.2025.108230

Date:

20-Feb-24 Release date: 05-Mar-25

Protein chain

A0MNG2  (A0MNG2_9CAUD) -  M27/M37 peptidase from Thermus phage phiYS40

Seq: 346 a.a.

Struc: 345 a.a.*

* PDB and UniProt seqs differ at 46 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.24.32 - beta-lytic metalloendopeptidase.
• Reaction: Cleavage of N-acetylmuramoyl-|-Ala, and of the insulin B chain at 23-Gly-|-Phe-24 > 18-Val-|-Cys(SO(3)H).
• Cofactor: Zn(2+)