PDBsum entry 8s1l

Hydrolase

PDB id: 8s1l

Contents

Protein chains

231 a.a.

316 a.a.

Ligands

CEI ×4

Waters ×93

PDB id:

8s1l

Name:

Hydrolase

Title:

Crystal structure of renilla reniformis luciferase rluc8-gfp bret complex at ph 6.0

Structure:

Green fluorescent protein. Chain: a, b, e, f. Engineered: yes. Coelenterazine h 2-monooxygenase. Chain: c, d, g, h. Synonym: renilla-luciferin 2-monooxygenase,renilla-type luciferase. Engineered: yes

Source:

Renilla reniformis. Sea pansy. Organism_taxid: 6136. Gene: gfp. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.40Å R-factor: 0.271 R-free: 0.340

Authors:

M.Marek,A.Smrckova

Key ref:

M.Marek and a.smrckova Crystal structure of renilla reniformis luciferase rl bret complex at ph 6.0. To be published, .

Date:

15-Feb-24 Release date: 03-Sep-25

Protein chains

Q963I9  (Q963I9_RENRE) -  Green fluorescent protein from Renilla reniformis

Seq: 233 a.a.

Struc: 231 a.a.*

Protein chains

P27652  (LUCI_RENRE) -  Coelenterazine h 2-monooxygenase from Renilla reniformis

Seq: 311 a.a.

Struc: 316 a.a.*

* PDB and UniProt seqs differ at 9 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains C, D, G, H: E.C.1.13.12.5 - Renilla-type luciferase.
• Pathway: Renilla-luciferin 2-monooxygenase; Watasenia-luciferin 2-monooxygenase; and Oplophorus-luciferin 2-monooxygenase
• Reaction: coelenterazine h + O2 = excited coelenteramide h monoanion + hnu + CO2 + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site