PDBsum entry 8s0e

Replication

PDB id

8s0e

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Contents

			Protein chains

					279 a.a.


					259 a.a.


					572 a.a.


					512 a.a.


					629 a.a.


					357 a.a.


					273 a.a.


					574 a.a.


					337 a.a.


					421 a.a.


					364 a.a.


					406 a.a.


					364 a.a.

			DNA/RNA

			Ligands

					AGS ×5

			Metals

					_MG ×5


					_ZN

PDB id:

8s0e

Links
- PDBe
- RCSB
- MMDB
- JenaLib
- Proteopedia
- CATH
- SCOP
- PDBSWS
- ProSAT

Name:

Replication

Title:

H. Sapiens occm bound to double stranded DNA

Structure:

DNA (39-mer). Chain: x. Engineered: yes. DNA (39-mer). Chain: y. Engineered: yes. DNA replication licensing factor mcm5. Chain: 5. Synonym: cdc46 homolog,p1-cdc46.

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Homo sapiens. Human. Organism_taxid: 9606. Gene: mcm5, cdc46. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Authors:

J.F.Greiwe,F.Weissmann,J.F.X.Diffley,A.Costa

Key ref:

F.Weissmann et al. Mcm double hexamer loading visualised with human prot. Nature, . PubMed id: 39604733

Date:

13-Feb-24

Release date:

02-Oct-24

PROCHECK

	Headers

	References

Protein chain

P33992 (MCM5_HUMAN) - DNA replication licensing factor MCM5 from Homo sapiens

Seq: Struc:

Seq: Struc:					734 a.a. 279 a.a.

Protein chain

Q9H211 (CDT1_HUMAN) - DNA replication factor Cdt1 from Homo sapiens

Seq: Struc:

Seq: Struc:					546 a.a. 259 a.a.

Protein chain

P49736 (MCM2_HUMAN) - DNA replication licensing factor MCM2 from Homo sapiens

Seq: Struc:

Seq: Struc:					904 a.a. 572 a.a.

Protein chain

P33991 (MCM4_HUMAN) - DNA replication licensing factor MCM4 from Homo sapiens

Seq: Struc:

Seq: Struc:					863 a.a. 512 a.a.*

Protein chain

Q14566 (MCM6_HUMAN) - DNA replication licensing factor MCM6 from Homo sapiens

Seq: Struc:

Seq: Struc:					821 a.a. 629 a.a.

Protein chain

P33993 (MCM7_HUMAN) - DNA replication licensing factor MCM7 from Homo sapiens

Seq: Struc:

Seq: Struc:					719 a.a. 357 a.a.

Protein chain

Q13416 (ORC2_HUMAN) - Origin recognition complex subunit 2 from Homo sapiens

Seq: Struc:

Seq: Struc:					577 a.a. 273 a.a.

Protein chain

Q9UBD5 (ORC3_HUMAN) - Origin recognition complex subunit 3 from Homo sapiens

Seq: Struc:

Seq: Struc:					711 a.a. 574 a.a.

Protein chain

Q99741 (CDC6_HUMAN) - Cell division control protein 6 homolog from Homo sapiens

Seq: Struc:

Seq: Struc:					560 a.a. 337 a.a.

Protein chain

P25205 (MCM3_HUMAN) - DNA replication licensing factor MCM3 from Homo sapiens

Seq: Struc:

Seq: Struc:					808 a.a. 421 a.a.

Protein chain

Q13415 (ORC1_HUMAN) - Origin recognition complex subunit 1 from Homo sapiens

Seq: Struc:

Seq: Struc:					861 a.a. 364 a.a.

Protein chain

O43929 (ORC4_HUMAN) - Origin recognition complex subunit 4 from Homo sapiens

Seq: Struc:					436 a.a. 406 a.a.

Protein chain

O43913 (ORC5_HUMAN) - Origin recognition complex subunit 5 from Homo sapiens

Seq: Struc:					435 a.a. 364 a.a.

Key:

Secondary structure

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

		G-C-A-T-G-C-A-T-G-C-A-T-G-C-T-G-C-A-T-G-C-A-T-G-C-A-T-G-C-G-C-A-T-G-C-A-T-G-C 39 bases
		G-C-A-T-G-C-A-T-G-C-G-C-A-T-G-C-A-T-G-C-A-T-G-C-A-G-C-A-T-G-C-A-T-G-C-A-T-G-C 39 bases



		Enzyme reactions

Enzyme class:

Chains 5, 2, 4, 6, 7, 3: E.C.3.6.4.12 - Dna helicase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

ATP + H2O = ADP + phosphate + H⁺

ATP
Bound ligand (Het Group name = AGS)
matches with 93.75% similarity

H2O

ADP

phosphate

H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site