PDBsum entry 8s09

Replication

PDB id: 8s09

Contents

Protein chains

692 a.a.

621 a.a.

623 a.a.

697 a.a.

703 a.a.

565 a.a.

DNA/RNA

Ligands

ATP ×2

ADP ×8

Metals

_ZN ×10

_MG ×8

PDB id:

8s09

Name:

Replication

Title:

H. Sapiens mcm2-7 double hexamer bound to double stranded DNA

Structure:

DNA (45-mer). Chain: x. Engineered: yes. DNA (45-mer). Chain: y. Engineered: yes. DNA replication licensing factor mcm2. Chain: 2, a. Synonym: minichromosome maintenance protein 2 homolog,nuclear protein

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Homo sapiens. Human. Organism_taxid: 9606. Gene: mcm2, bm28, ccnl1, cdcl1, kiaa0030. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Authors:

J.F.Greiwe,F.Weissmann,J.F.X.Diffley,A.Costa

Key ref:

F.Weissmann et al. Mcm double hexamer loading visualised with human prot. Nature, . PubMed id: 39604733

Date:

13-Feb-24 Release date: 02-Oct-24

Protein chains

P49736  (MCM2_HUMAN) -  DNA replication licensing factor MCM2 from Homo sapiens

Seq: 904 a.a.

Struc: 692 a.a.

Protein chains

P25205  (MCM3_HUMAN) -  DNA replication licensing factor MCM3 from Homo sapiens

Seq: 808 a.a.

Struc: 621 a.a.

Protein chains

P33991  (MCM4_HUMAN) -  DNA replication licensing factor MCM4 from Homo sapiens

Seq: 863 a.a.

Struc: 623 a.a.*

Protein chains

P33992  (MCM5_HUMAN) -  DNA replication licensing factor MCM5 from Homo sapiens

Seq: 734 a.a.

Struc: 697 a.a.

Protein chains

Q14566  (MCM6_HUMAN) -  DNA replication licensing factor MCM6 from Homo sapiens

Seq: 821 a.a.

Struc: 703 a.a.

Protein chains

P33993  (MCM7_HUMAN) -  DNA replication licensing factor MCM7 from Homo sapiens

Seq: 719 a.a.

Struc: 565 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-C-A-T-G-C-A-T-G-C-G-C-A-T-G-C-A-T-G-C-A-T-T-A-T-G-C-A-T-G-C-A-T-G-C-G-C-A-T- 45 bases

G-C-A-T-G-C-A-T-G-C-G-C-A-T-G-C-A-T-G-C-A-T-A-A-T-G-C-A-T-G-C-A-T-G-C-G-C-A-T- 45 bases

Enzyme reactions

• Enzyme class: Chains 2, 3, 4, 5, 6, 7, A, B, C, D, E, F: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP (Bound ligand (Het Group name = ATP) corresponds exactly) + H2O = ADP + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site