PDBsum entry 8qw6

Oncoprotein

PDB id: 8qw6

Contents

Protein chains

104 a.a.

86 a.a.

144 a.a.

155 a.a.

163 a.a.

Ligands

GDP ×2

X4R ×2

Metals

_MG ×2

Waters ×48

PDB id:

8qw6

Name:

Oncoprotein

Title:

Crystal structure of compound 3 in complex with kras g12v c118s gdp and pvhl:elonginc:elonginb

Structure:

Elongin-b. Chain: h, d. Synonym: elob,elongin 18 kda subunit,RNA polymerase ii transcription factor siii subunit b,siii p18,transcription elongation factor b polypeptide 2. Engineered: yes. Mutation: yes. Elongin-c. Chain: g, c.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: elob, tceb2. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: eloc, tceb1. Gene: vhl. Gene: kras, kras2, rask2.

Resolution:

2.20Å R-factor: 0.257 R-free: 0.295

Authors:

D.Zollman,W.Farnaby,A.Ciulli

Key ref:

J.Popow et al. Targeting cancer with small molecule pan-Kras degrade. Biorxiv, . PubMed id: 39298590

Date:

18-Oct-23 Release date: 06-Dec-23

Protein chains

Q15370  (ELOB_HUMAN) -  Elongin-B from Homo sapiens

Seq: 118 a.a.

Struc: 104 a.a.

Protein chains

Q15369  (ELOC_HUMAN) -  Elongin-C from Homo sapiens

Seq: 112 a.a.

Struc: 86 a.a.

Protein chains

P40337  (VHL_HUMAN) -  von Hippel-Lindau disease tumor suppressor from Homo sapiens

Seq: 213 a.a.

Struc: 144 a.a.

Protein chain

P01116  (RASK_HUMAN) -  GTPase KRas from Homo sapiens

Seq: 189 a.a.

Struc: 155 a.a.*

Protein chain

P01116  (RASK_HUMAN) -  GTPase KRas from Homo sapiens

Seq: 189 a.a.

Struc: 163 a.a.*

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains E, A: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP + H2O = GDP (Bound ligand (Het Group name = GDP) corresponds exactly) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site