PDBsum entry 8qos

Unknown function

PDB id: 8qos

Contents

Protein chains

(+ 0 more) 131 a.a.

Ligands

EDO ×5

Metals

__K ×3

Waters ×627

PDB id:

8qos

Name:

Unknown function

Title:

Capra hircus reactive intermediate deaminase a - v25w

Structure:

2-iminobutanoate/2-iminopropanoate deaminase. Chain: a, b, c, d, e, f. Engineered: yes

Source:

Capra hircus. Goat. Organism_taxid: 9925. Gene: rida. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.01Å R-factor: 0.240 R-free: 0.285

Authors:

G.Rizzi,C.Visentin,F.Di Pisa,S.Ricagno

Key ref:

G.Rizzi et al. Capra hircus reactive intermediate deaminase a - V25w. To be published, . PubMed id: 38801230

Date:

29-Sep-23 Release date: 26-Jun-24

Protein chains

P80601  (RIDA_CAPHI) -  2-iminobutanoate/2-iminopropanoate deaminase from Capra hircus

Seq: 137 a.a.

Struc: 131 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.3.1.-.-
• Enzyme class 3: E.C.3.5.99.10 - 2-iminobutanoate/2-iminopropanoate deaminase.
• Reaction:
  1. 2-iminobutanoate + H2O = 2-oxobutanoate + NH4⁺
  2. 2-iminopropanoate + H2O = pyruvate + NH4⁺

2-iminobutanoate + H2O = 2-oxobutanoate (Bound ligand (Het Group name = EDO) matches with 57.14% similarity) + NH4(+)

2-iminopropanoate + H2O = pyruvate (Bound ligand (Het Group name = EDO) matches with 66.67% similarity) + NH4(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site