PDBsum entry 8qhc

RNA binding protein

PDB id: 8qhc

Contents

Protein chains

393 a.a.

1183 a.a.

188 a.a.

DNA/RNA

Ligands

GTP

Metals

_MG

PDB id:

8qhc

Name:

RNA binding protein

Title:

Cryo-em structure of sidh from legionella pneumophila in complex with lubx

Structure:

Elongation factor tu. Chain: c. T-RNA. Chain: b. Protein sidh. Chain: a. Engineered: yes. E3 ubiquitin--protein ligase. Chain: d.

Source:

Escherichia coli 'bl21-gold(de3)plyss ag'. Organism_taxid: 866768. Legionella pneumophila. Organism_taxid: 446. Gene: c3927_14265. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768. Gene: c3927_14270. Expressed in: escherichia coli.

Authors:

R.Sharma,M.Adams,S.Bhogaraju

Key ref:

R.Sharma et al. Structural basis for the toxicity of legionella pneum effector sidh. To be published, . PubMed id: 37923743

Date:

07-Sep-23 Release date: 11-Oct-23

Protein chain

E2QJ06  (E2QJ06_ECOLX) -  Elongation factor Tu from Escherichia coli

Seq: 394 a.a.

Struc: 393 a.a.

Protein chain

Q6RCQ4  (Q6RCQ4_LEGPN) -  SidH from Legionella pneumophila

Seq: 2225 a.a.

Struc: 1183 a.a.

Protein chain

A0A2S6F0E3  (A0A2S6F0E3_LEGPN) -

DNA/RNA chain

G-C-C-C-G-G-A-4SU-A-G-C-U-C-A-G-H2U-C-G-G-H2U-A-G-A-G-C-A-G-C-G-U-G-3AU-C-C-U- 61 bases

Enzyme reactions

• Enzyme class: Chain D: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine