PDBsum entry 8q1h

Oxidoreductase

PDB id: 8q1h

Contents

Protein chains

666 a.a.

130 a.a.

16 a.a.

Ligands

FAD

PDB id:

8q1h

Name:

Oxidoreductase

Title:

Lsd1 y391k-corest bound to histone h3 n-terminal tail

Structure:

Lysine-specific histone demethylase 1a. Chain: a. Engineered: yes. Rest corepressor 1. Chain: b. Synonym: protein corest. Engineered: yes. Histone h3.3c. Chain: c.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: kdm1a. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Gene: rcor1, kiaa0071, rcor. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.90Å R-factor: 0.233 R-free: 0.254

Authors:

M.Barone,A.Mattevi

Key ref:

L.Kwangwoon et al. Uncoupling histone modification crosstalk by engineer lysine demethylase lsd1. To be published, . PubMed id: 38965385

Date:

31-Jul-23 Release date: 15-May-24

Protein chain

O60341  (KDM1A_HUMAN) -  Lysine-specific histone demethylase 1A from Homo sapiens

Seq: 852 a.a.

Struc: 666 a.a.*

Protein chain

Q9UKL0  (RCOR1_HUMAN) -  REST corepressor 1 from Homo sapiens

Seq: 485 a.a.

Struc: 130 a.a.

Protein chain

Q6NXT2  (H3C_HUMAN) -  Histone H3.3C from Homo sapiens

Seq: 135 a.a.

Struc: 16 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.1.14.99.66 - [histone-H3]-N(6),N(6)-dimethyl-L-lysine(4) FAD-dependent demethylase.
• Reaction: N₆,N₆-dimethyl-L-lysyl₄-[histone H3] + 2 A + 2 H2O = L-lysyl₄- [histone H3] + 2 formaldehyde + 2 AH2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site