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PDBsum entry 8prv
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Cytosolic protein
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PDB id
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8prv
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Contents |
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1584 a.a.
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163 a.a.
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2034 a.a.
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PDB id:
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| Name: |
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Cytosolic protein
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Title:
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Asymmetric unit of the yeast fatty acid synthase in the non-rotated state with acp at the ketosreductase domain (fasamn sample)
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Structure:
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Fatty acid synthase subunit alpha. Chain: a, b. Fatty acid synthase subunit beta. Chain: g. Ec: 2.3.1.86,4.2.1.59,1.3.1.9,2.3.1.38,2.3.1.39,3.1.2.14
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Source:
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Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Organism_taxid: 4932
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Authors:
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K.Singh,G.Bunzel,B.Graf,K.M.Yip,H.Stark,A.Chari
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Key ref:
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K.Singh
et al.
(2023).
Reconstruction of a fatty acid synthesis cycle from a carrier protein and cofactor structural snapshots..
Cell,
186,
5054.
PubMed id:
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Date:
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12-Jul-23
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Release date:
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22-Nov-23
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PROCHECK
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Headers
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References
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P19097
(FAS2_YEAST) -
Fatty acid synthase subunit alpha from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Seq:
Struc:
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1887 a.a.
1584 a.a.
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Enzyme class 2:
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Chains A, B:
E.C.1.1.1.100
- 3-oxoacyl-[acyl-carrier-protein] reductase.
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Reaction:
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a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + NADPH + H+
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(3R)-hydroxyacyl-[ACP]
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+
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NADP(+)
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=
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3-oxoacyl-[ACP]
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+
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NADPH
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+
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H(+)
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Enzyme class 3:
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Chains A, B:
E.C.2.3.1.41
- beta-ketoacyl-[acyl-carrier-protein] synthase I.
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Reaction:
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a fatty acyl-[ACP] + malonyl-[ACP] + H+ = a 3-oxoacyl-[ACP] + holo- [ACP] + CO2
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fatty acyl-[ACP]
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+
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malonyl-[ACP]
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+
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H(+)
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=
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3-oxoacyl-[ACP]
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+
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holo- [ACP]
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+
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CO2
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Enzyme class 4:
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Chains A, B, G:
E.C.2.3.1.86
- fatty-acyl-CoA synthase system.
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Reaction:
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acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H+ = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+
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acetyl-CoA
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+
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n
malonyl-CoA
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+
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2n NADPH
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+
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4n H(+)
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=
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long-chain-acyl-CoA
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+
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n
CoA
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+
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n
CO2
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+
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2n NADP(+)
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Enzyme class 5:
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Chain G:
E.C.1.3.1.9
- enoyl-[acyl-carrier-protein] reductase (NADH).
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Reaction:
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a 2,3-saturated acyl-[ACP] + NAD+ = a (2E)-enoyl-[ACP] + NADH + H+
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2,3-saturated acyl-[ACP]
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+
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n
NAD(+)
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=
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(2E)-enoyl-[ACP]
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+
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NADH
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+
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H(+)
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Enzyme class 6:
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Chain G:
E.C.2.3.1.38
- [acyl-carrier-protein] S-acetyltransferase.
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Reaction:
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holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA
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holo-[ACP]
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+
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n
acetyl-CoA
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=
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acetyl-[ACP]
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+
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CoA
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Enzyme class 7:
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Chain G:
E.C.2.3.1.39
- [acyl-carrier-protein] S-malonyltransferase.
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Reaction:
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holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA
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holo-[ACP]
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+
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n
malonyl-CoA
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=
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malonyl-[ACP]
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+
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CoA
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Enzyme class 8:
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Chain G:
E.C.3.1.2.14
- oleoyl-[acyl-carrier-protein] hydrolase.
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Reaction:
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(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H+
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Oleoyl-[acyl-carrier-protein]
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+
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n
H(2)O
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=
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[acyl-carrier-protein]
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+
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oleate
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Enzyme class 9:
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Chain G:
E.C.4.2.1.59
- 3-hydroxyacyl-[acyl-carrier-protein] dehydratase.
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Reaction:
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a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
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(3R)-3-hydroxyacyl-[acyl-carrier protein]
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=
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n
trans-2-enoyl-[acyl- carrier protein]
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+
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H(2)O
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
