PDBsum entry 8pc9

Hydrolase

PDB id: 8pc9

Contents

Protein chain

261 a.a.

Ligands

SO4

Waters ×236

PDB id:

8pc9

Name:

Hydrolase

Title:

Structure of serine-beta-lactamase ctx-m-14 following the time- resolved active site binding of boric acid, 0 ms - native.

Structure:

Beta-lactamase. Chain: a. Engineered: yes

Source:

Klebsiella pneumoniae. Organism_taxid: 573. Gene: ctx-m-14. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.40Å R-factor: 0.146 R-free: 0.170

Authors:

A.Prester,M.Perbandt,M.Galchenkova,D.Oberthuer,O.Yefanov,W.Hinrichs, H.Rohde,C.Betzel

Key ref:

A.Prester et al. Time resolved crystallography of boric acid binding t active site serine of the beta-Lactamase ctx-M-14 and subsequent 1,2-Diol esterification. To be published, . PubMed id: 38969718

Date:

11-Jun-23 Release date: 26-Jun-24

Protein chain

A0A0H3H219  (A0A0H3H219_KLEPH) -  Beta-lactamase from Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)

Seq: 291 a.a.

Struc: 261 a.a.

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)