PDBsum entry 8p4c

Transcription

PDB id: 8p4c

Contents

Protein chains

1410 a.a.

493 a.a.

1132 a.a.

263 a.a.

209 a.a.

82 a.a.

148 a.a.

117 a.a.

67 a.a.

115 a.a.

46 a.a.

126 a.a.

171 a.a.

116 a.a.

458 a.a.

DNA/RNA

Metals

_MG

_ZN ×3

PDB id:

8p4c

Name:

Transcription

Title:

Structural insights into human co-transcriptional capping - structure 3

Structure:

DNA-directed RNA polymerase ii subunit rpb1. Chain: a. mRNA-capping enzyme. Chain: m. Synonym: hcap1,hce. Engineered: yes. DNA (32-mer). Chain: n. Engineered: yes.

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Homo sapiens. Human. Organism_taxid: 9606. Gene: rngtt, cap1a. Expressed in: escherichia coli. Expression_system_taxid: 562.

Authors:

G.Garg,C.Dienemann,L.Farnung,J.Schwarz,A.Linden,H.Urlaub,P.Cramer

Key ref:

G.Garg et al. (2023). Structural insights into human co-Transcriptional cap. Mol cell, 83, 2464. PubMed id: 37369200

Date:

20-May-23 Release date: 16-Aug-23

Protein chain

A0A8D1DPV6  (A0A8D1DPV6_PIG) -  DNA-directed RNA polymerase subunit from Sus scrofa

Seq: 1970 a.a.

Struc: 1410 a.a.

Protein chain

O60942  (MCE1_HUMAN) -  mRNA-capping enzyme from Homo sapiens

Seq: 597 a.a.

Struc: 493 a.a.

Protein chain

I3LGP4  (I3LGP4_PIG) -  DNA-directed RNA polymerase subunit beta from Sus scrofa

Seq: 1300 a.a.

Struc: 1132 a.a.

Protein chain

I3LCH3  (I3LCH3_PIG) -  DNA-directed RNA polymerase II subunit RPB3 from Sus scrofa

Seq: 275 a.a.

Struc: 263 a.a.

Protein chain

I3LSI7  (I3LSI7_PIG) -  DNA-directed RNA polymerase II subunit E from Sus scrofa

Seq: 210 a.a.

Struc: 209 a.a.

Protein chain

A0A4X1VEK9  (A0A4X1VEK9_PIG) -  DNA-directed RNA polymerases I, II, and III subunit RPABC2 from Sus scrofa

Seq: 127 a.a.

Struc: 82 a.a.

Protein chain

I3LCB2  (I3LCB2_PIG) -  DNA-directed RNA polymerases I, II, and III subunit RPABC3 from Sus scrofa

Seq: 150 a.a.

Struc: 148 a.a.

Protein chain

P60899  (RPB9_PIG) -  DNA-directed RNA polymerase II subunit RPB9 from Sus scrofa

Seq: 125 a.a.

Struc: 117 a.a.

Protein chain

A0A8W4F9W9  (A0A8W4F9W9_PIG) -  DNA-directed RNA polymerases I, II, and III subunit RPABC5 from Sus scrofa

Seq: 67 a.a.

Struc: 67 a.a.

Protein chain

F1RKE4  (F1RKE4_PIG) -  DNA-directed RNA polymerase II subunit RPB11-a from Sus scrofa

Seq: 117 a.a.

Struc: 115 a.a.

Protein chain

A0A4X1TRS6  (A0A4X1TRS6_PIG) -  RNA polymerase II, I and III subunit K from Sus scrofa

Seq: 58 a.a.

Struc: 46 a.a.

Protein chain

A0A4X1VM56  (A0A4X1VM56_PIG) -  RNA polymerase II subunit D from Sus scrofa

Seq: 142 a.a.

Struc: 126 a.a.

Protein chain

A0A4X1VKG7  (A0A4X1VKG7_PIG) -  DNA-directed RNA polymerase subunit from Sus scrofa

Seq: 172 a.a.

Struc: 171 a.a.

Protein chain

P63272  (SPT4H_HUMAN) -  Transcription elongation factor SPT4 from Homo sapiens

Seq: 117 a.a.

Struc: 116 a.a.

Protein chain

O00267  (SPT5H_HUMAN) -  Transcription elongation factor SPT5 from Homo sapiens

Seq: 1087 a.a.

Struc: 458 a.a.

DNA/RNA chains

C-T-A-G-T-A-C-C-T-A-G-C-T-T-A-A-G-C-C-T-C-A-A-T-A-A-A-G-C-T-T-G 32 bases

C-A-A-G-C-T-T-T-A-T-T-G-A-G-G-C-T-T-A-A-G-C-A-G-T-G-G-G-T-T-C-C-A-G-G-T-A-C-T- 41 bases

C-G-G-A-G-A-G-G-G-A-A-C-C-C-A-C-U 17 bases

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: Chain M: E.C.2.7.7.50 - mRNA guanylyltransferase.
• Reaction: a 5'-end diphospho-ribonucleoside in mRNA + GTP + H⁺ = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate
• Enzyme class 4: Chain M: E.C.3.6.1.74 - mRNA 5'-phosphatase.
• Reaction: a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho- ribonucleoside in mRNA + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site