PDBsum entry 8ovh

Lyase

PDB id: 8ovh

Contents

Protein chains

400 a.a.

Ligands

PLP ×4

PEG ×4

Waters ×482

PDB id:

8ovh

Name:

Lyase

Title:

Crystal structure of o-acetyl-l-homoserine sulfhydrolase from saccharomyces cerevisiae in complex with pyridoxal-5'-phosphate

Structure:

Homocysteine/cysteine synthase. Chain: a, b, c, d. Synonym: o-acetylserine/o-acetylhomoserine sulfhydrylase,oas-oah shlase,oas-oah sulfhydrylase. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: met17, met15, met25, ylr303w, l8003.1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.17Å R-factor: 0.172 R-free: 0.238

Authors:

R.Saleem-Batcha,J.N.Andexer,M.Mohr

Key ref:

M.K.F.Mohr et al. Enzymatic synthesis of l-Methionine analogues and application in a methyltransferase catalysed alkylati cascade.. Chemistry, . PubMed id: 37235813

Date:

26-Apr-23 Release date: 21-Jun-23

Protein chains

P06106  (CYSD_YEAST) -  Homocysteine/cysteine synthase from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 444 a.a.

Struc: 400 a.a.

Enzyme reactions

• Enzyme class 1: E.C.2.5.1.47 - cysteine synthase.
• Reaction: O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

O-acetyl-L-serine + hydrogen sulfide (Bound ligand (Het Group name = PEG) matches with 41.67% similarity) = L-cysteine + acetate

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

• Enzyme class 2: E.C.2.5.1.49 - O-acetylhomoserine aminocarboxypropyltransferase.
• Reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site