PDBsum entry 8or0

Ligase

PDB id: 8or0

Contents

Protein chains

674 a.a.

88 a.a.

1137 a.a.

134 a.a.

307 a.a.

64 a.a.

85 a.a.

Metals

_ZN ×3

PDB id:

8or0

Name:

Ligase

Title:

Cand1-cul1-rbx1-skp1-skp2-cks1-cdk2

Structure:

Cullin-1. Chain: a. Synonym: cul-1. Engineered: yes. E3 ubiquitin-protein ligase rbx1. Chain: b. Synonym: e3 ubiquitin-protein transferase rbx1,protein zyp,ring finger protein 75,ring-box protein 1,rbx1,regulator of cullins 1, roc1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cul1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: rbx1, rnf75, roc1. Gene: cand1, kiaa0829, tip120, tip120a. Expressed in: escherichia coli bl21(de3).

Authors:

M.Shaaban,J.A.Clapperton,S.Ding,M.E.Maeots,R.I.Enchev

Key ref:

M.Shaaban et al. Structural and mechanistic insights into the cand1-Me scf substrate receptor exchange.. Mol cell, . PubMed id: 37339624

Date:

12-Apr-23 Release date: 28-Jun-23

Protein chain

Q13616  (CUL1_HUMAN) -  Cullin-1 from Homo sapiens

Seq: 776 a.a.

Struc: 674 a.a.

Protein chain

P62877  (RBX1_HUMAN) -  E3 ubiquitin-protein ligase RBX1 from Homo sapiens

Seq: 108 a.a.

Struc: 88 a.a.

Protein chain

Q86VP6  (CAND1_HUMAN) -  Cullin-associated NEDD8-dissociated protein 1 from Homo sapiens

Seq: 1230 a.a.

Struc: 1137 a.a.

Protein chain

P63208  (SKP1_HUMAN) -  S-phase kinase-associated protein 1 from Homo sapiens

Seq: 163 a.a.

Struc: 134 a.a.

Protein chain

Q13309  (SKP2_HUMAN) -  S-phase kinase-associated protein 2 from Homo sapiens

Seq: 424 a.a.

Struc: 307 a.a.

Protein chain

P61024  (CKS1_HUMAN) -  Cyclin-dependent kinases regulatory subunit 1 from Homo sapiens

Seq: 79 a.a.

Struc: 64 a.a.

Protein chain

P24941  (CDK2_HUMAN) -  Cyclin-dependent kinase 2 from Homo sapiens

Seq: 298 a.a.

Struc: 85 a.a.

Enzyme reactions

• Enzyme class 1: Chain B: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 2: Chain B: E.C.2.3.2.32 - cullin-RING-type E3 NEDD8 transferase.
• Reaction: S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]- L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N₆-[NEDD8- protein]-yl-[cullin]-L-lysine
• Enzyme class 3: Chain H: E.C.2.7.11.22 - cyclin-dependent kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site