PDBsum entry 8ojc

Transferase

PDB id: 8ojc

Contents

Protein chain

395 a.a.

DNA/RNA

Metals

_CA ×2

Waters ×288

PDB id:

8ojc

Name:

Transferase

Title:

Hsv-1 DNA polymerase active site in alternative exonuclease state

Structure:

DNA (47-mer). Chain: c. Engineered: yes. DNA polymerase catalytic subunit. Chain: a. Engineered: yes

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Human alphaherpesvirus 1 strain kos. Organism_taxid: 10306. Gene: ul30. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Authors:

E.Gustavsson,K.Grunewald,P.Elias,B.M.Hallberg

Key ref:

E.Gustavsson et al. Dynamics of the herpes simplex virus DNA polymerase holoenzyme during DNA synthesis and proof-Reading rev by cryo-Em. To be published, . PubMed id: 38806233

Date:

24-Mar-23 Release date: 03-Apr-24

Protein chain

P04293  (DPOL_HHV11) -  DNA polymerase catalytic subunit from Human herpesvirus 1 (strain 17)

Seq: 1235 a.a.

Struc: 395 a.a.

DNA/RNA chain

A-A-_AS 3 bases

Enzyme reactions

• Enzyme class 2: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.1.26.4 - ribonuclease H.
• Reaction: Endonucleolytic cleavage to 5'-phosphomonoester.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site