PDBsum entry 8k5a

Transcription/DNA/RNA

PDB id: 8k5a

Contents

Protein chains

230 a.a.

1340 a.a.

1352 a.a.

76 a.a.

129 a.a.

DNA/RNA

PDB id:

8k5a

Name:

Transcription/DNA/RNA

Title:

The cryo-em map of open tiea-tec complex

Structure:

DNA-directed RNA polymerase subunit alpha. Chain: a, b. Synonym: rnap subunit alpha,RNA polymerase subunit alpha, transcriptase subunit alpha. Engineered: yes. DNA-directed RNA polymerase subunit beta. Chain: c. Synonym: rnap subunit beta,RNA polymerase subunit beta,transcriptase subunit beta.

Source:

Escherichia coli k-12. Organism_taxid: 83333. Gene: rpoa, pez, phs, sez, b3295, jw3257. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: rpob, gron, nitb, rif, ron, stl, stv, tabd, b3987, jw3950. Gene: rpoc, tabb, b3988, jw3951. Gene: rpoz, b3649, jw3624. Escherichia phage t4.

Authors:

K.N.Zhang,Y.Liu,M.Chen,Y.Wang,W.Lin,M.Li,X.Zhang,Y.Gao,Q.Gong,H.Chen, M.Steve,S.Li,K.Zhang,B.Liu

Key ref:

K.N.Zhang et al. Tiea inhibits sigma70-Dependent transcriptions, Accel elongation speed and elevates transcription error. To be published, .

Date:

21-Jul-23 Release date: 24-Jul-24

Protein chains

P0A7Z4  (RPOA_ECOLI) -  DNA-directed RNA polymerase subunit alpha from Escherichia coli (strain K12)

Seq: 329 a.a.

Struc: 230 a.a.

Protein chain

P0A8V2  (RPOB_ECOLI) -  DNA-directed RNA polymerase subunit beta from Escherichia coli (strain K12)

Seq: 1342 a.a.

Struc: 1340 a.a.

Protein chain

P0A8T7  (RPOC_ECOLI) -  DNA-directed RNA polymerase subunit beta' from Escherichia coli (strain K12)

Seq: 1407 a.a.

Struc: 1352 a.a.

Protein chain

P0A800  (RPOZ_ECOLI) -  DNA-directed RNA polymerase subunit omega from Escherichia coli (strain K12)

Seq: 91 a.a.

Struc: 76 a.a.

Protein chain

P07879  (RPBA_BPT4) -  15 kDa RNA polymerase-binding protein from Enterobacteria phage T4

Seq: 129 a.a.

Struc: 129 a.a.

DNA/RNA chains

G-G-G-T-A-T-T-C-G-C-C-G-T-G-T-A-C-C-T-C-T-C-C-T-A-G-C-C-C 29 bases

G-G-G-C-T-A-A-T-G-A-C-G-G-C-G-A-A-T-A-C-C-C 22 bases

C-G-G-A-G-A-G-G-U-A 10 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site