PDBsum entry 8id2

RNA binding protein/RNA

PDB id: 8id2

Contents

Protein chains

95 a.a.

89 a.a.

DNA/RNA

Waters ×100

PDB id:

8id2

Name:

RNA binding protein/RNA

Title:

Crystal structure of the ubiquitin-like domain in the sf3a1 subunit of human u2 snrnp complexed with the stem-loop 4 of u1 snrna

Structure:

Splicing factor 3a subunit 1. Chain: a, b. Synonym: sf3a120,spliceosome-associated protein 114,sap 114. Engineered: yes. RNA (5'- r( Gp Gp Gp Gp Ap Cp Up Gp Cp Gp Up Up Cp Gp Cp Gp Cp Up Up Up Cp Cp Cp C)-3'). Chain: c, d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: sf3a1, sap114. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.80Å R-factor: 0.197 R-free: 0.237

Authors:

S.Terawaki,N.Nameki,K.Kuwasako

Key ref:

N.Nameki et al. Structural insights into recognition of sl4, The uucg stem-Loop, Of human u1 snrna by the ubiquitin-Like do including the c-Terminal tail in the sf3a1 subunit of snrnp.. J biochem, . PubMed id: 37094335

Date:

12-Feb-23 Release date: 17-May-23

Protein chain

Q15459  (SF3A1_HUMAN) -  Splicing factor 3A subunit 1 from Homo sapiens

Seq: 793 a.a.

Struc: 95 a.a.*

Protein chain

Q15459  (SF3A1_HUMAN) -  Splicing factor 3A subunit 1 from Homo sapiens

Seq: 793 a.a.

Struc: 89 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

DNA/RNA chains

G-G-G-G-A-C-U-G-C-G-U-U-C-G-C-G-C-U-U-U-C-C-C-C 24 bases

G-G-G-G-A-C-U-G-C-G-U-U-C-G-C-G-C-U-U-U-C-C-C-C 24 bases

Enzyme reactions

• Enzyme class: Chains A, B: E.C.?