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PDBsum entry 8ibm
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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
8ibm

 

 

 

 

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Contents
Protein chains
262 a.a.
Ligands
SO4 ×2
Metals
_CA ×2
Waters ×61
PDB id:
8ibm
Name: Hydrolase
Title: Sulfate bound form of pet-degrading cutinase cut190 with thermostability-improving mutations of s226p/r228s/q138a/d250c- e296c/q123h/n202h and s176a inactivation
Structure: Alpha/beta hydrolase family protein. Chain: a, b. Synonym: cutinase. Engineered: yes. Mutation: yes
Source: Saccharomonospora viridis. Organism_taxid: 1852. Gene: cut190, samn02982918_2340. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.20Å     R-factor:   0.235     R-free:   0.267
Authors: M.Emori,N.Numoto,N.Kamiya,M.Oda
Key ref: N.Numoto et al. Improvement of thermostability and activity of pet-De enzyme cut190 towards a detailed understanding and application of the enzymatic reaction mechanism.. Biorxiv, .
Date:
10-Feb-23     Release date:   15-Mar-23    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
W0TJ64  (W0TJ64_9PSEU) -  cutinase from Saccharomonospora viridis
Seq:
Struc: 		304 a.a.
262 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 9 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.1.1.74  - cutinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: cutin + H2O = cutin monomers

 

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