PDBsum entry 8i1h

Hydrolase

PDB id: 8i1h

Contents

Protein chains

153 a.a.

Ligands

GOL ×2

Metals

_NA ×3

Waters ×347

PDB id:

8i1h

Name:

Hydrolase

Title:

Crystal structure of human mth1(g2k mutant) in complex with 2-oxo-datp at ph 9.7

Structure:

7,8-dihydro-8-oxoguanine triphosphatase. Chain: a, b. Fragment: unp residues 42-197. Synonym: mutt homologue-1,2-hydroxy-datp diphosphatase,8-oxo-dgtpase, nucleoside diphosphate-linked moiety x motif 1,nudix motif 1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: nudt1, mth1. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.18Å R-factor: 0.137 R-free: 0.166

Authors:

T.Nakamura,Y.Yamagata

Key ref:

T.Nakamura et al. Protonation states of asp residues in the human nudix hydrolase mth1 contribute to its broad substrate recognition.. Febs lett, . PubMed id: 36914375

Date:

13-Jan-23 Release date: 22-Mar-23

Protein chains

P36639  (8ODP_HUMAN) -  Oxidized purine nucleoside triphosphate hydrolase from Homo sapiens

Seq: 156 a.a.

Struc: 153 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.3.6.1.- - ?????
• Enzyme class 3: E.C.3.6.1.56 - 2-hydroxy-dATP diphosphatase.
• Reaction: 2-oxo-dATP + H2O = 2-oxo-dAMP + diphosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site